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This includes free shipping for all items with no minimum order value required. Items may be shipped and delivered at different times. You may get more than one tracking number if your order contains more than one item. You will receive a tracking number after your order is processed and ready to ship. Occasionally, during large promotional events and the holidays, more time may be required to validate and ship your order, causing a delay. We strive to ship the complete order for your convenience, but to ensure you get your order on time, the items may be separated. If your order contains more than one item, you may get more than one tracking number. We do not ship internationally or to US Territories (Caribbean, Puerto Rico, Canada and beyond). PO BOX and APO/FPO can be shipped via USPS Ground only. Review the Material Safety Data Sheet before handling.We ship to most locations within the United States (50 states as well as to Military (APO/FPO) addresses. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material.
High Five™ cells can be adapted to suspension culture.Įach lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.Ĭaution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual.
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Good growth in adherent or suspension culture They consistently express 5–10 times more protein that Sf9 cells. High Five™ cells are the best choice for expression of secreted protein in insect cells.
Up to ten-fold increase in secreted protein expression compared to Sf9 cells High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System. Recombinant protein expression from a variety of expression systems